Summary
Type: SPE
Application #: 303920
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection: LC/MS with RP column (e.g. EC 250/2 NUCLEODUR® C18 Gravity, 5 µm, MN Cat. No. 760100.20):
Mobile phase: Eluent A: 0.005 M ammonium formiat / NH3, pH 8.5; Eluent B: acetonitrile; Gradient: 25% B for 1
Conditions: Column conditioning: 4 ml methanol, then 4 ml water
Sample application: Applying of 4 ml sample extract
Column washing: 1 ml water, then 4 ml methanol / water (1:10)
Elution: 3 ml methanol / water (1:1).
Authors: Helle N TeLA GmbH Bremerhaven Germany
Source: Labor Praxis April 2006 52-55